Generator
Part:BBa_K2014001:Design
Designed by: Przemyslaw Nuc Group: iGEM16_UAM_Poznan (2016-10-11)
prha1-E15'UTR->htsfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 217
Design Notes
We were looking for the shortest, functional rhamnose induced promoter and for the best performing and universal 5'UTR sequence for several promoters of our multipromoter expression system.
Source
Rhamnose induced promoter is a modified part of Escherichia coli genome. 5'UTR is synthetic, similar to a transcript of T7 bacteriophage,sfGFP is synthetic, based on the publication of Jean-Denis Pedelacq et all (2006).