Generator

Part:BBa_K2014001:Design

Designed by: Przemyslaw Nuc   Group: iGEM16_UAM_Poznan   (2016-10-11)


prha1-E15'UTR->htsfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 217


Design Notes

We were looking for the shortest, functional rhamnose induced promoter and for the best performing and universal 5'UTR sequence for several promoters of our multipromoter expression system.


Source

Rhamnose induced promoter is a modified part of Escherichia coli genome. 5'UTR is synthetic, similar to a transcript of T7 bacteriophage,sfGFP is synthetic, based on the publication of Jean-Denis Pedelacq et all (2006).

References